Figure 9. JCTH-4 and CC do not dissipate MMP in HOb and NFF cells.
Effect of JCTH-4 and CC on MMP in (A) HOb and (B) NFF cells after 72 hours of treatment was examined by TMRM staining. Cells were grown on coverslips, treated with the indicated concentrations of JCTH-4, CC, and solvent control (Me2SO), and stained with TMRM and Hoechst dye. Images were taken at 400× magnification on a fluorescent microscope. Red fluorescent punctuate marks are indicative of mitochondria with intact MMP. Scale bar = 15 µm. All images are representative of 3 independent experiments.