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. 2011 Dec 21;6(12):e28780. doi: 10.1371/journal.pone.0028780

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Ma et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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MDC staining was used to detect the presence of autophagic vacuoles in (A) HOb and (B) NFF cells after 72 hours of treatment with JCTH-4, CC, and solvent control (Me₂SO) at the indicated concentrations. Bright blue punctate marks are indicative of autophagic vacuoles. Corresponding phase and PI micrographs are shown below the MDC images. Scale bar = 15 µm. All images are representative of 3 independent experiments.