Figure 1. Experimental inoculation of porcine gut and gut loops.

A) Injection sites of Entamoeba histolytica in the pig digestive tract. A total of 1–3 10⁶ virulent trophozoites in 10 ml TY-1-S-33 medium were injected at three different locations (cecum, ileocecal junction and proximal colon). Four animals were used. B) Schematic representation of jejunal loops. Three loops were surgically created in a 2–4 m section of the pig jejunum. Proximal loop was inoculated with 1 10⁶ virulent trophozoites in 10 ml TY-1-S-33 medium while distal loops were inoculated only with 10 ml medium. Besides isolated loops intestinal flow from duodenum to jejunum and ileum was maintained in the gut via an end-to-end anastomosis. Blood and lymph flow to the loops were preserved. A total of 9 pigs were used for gut loops.