Figure 2. Evaluation of the sensitivity and specificity of two Mp1p antigen ELISAs.

(A, B): evaluation of the sensitivity by detection of rMp1p and natural Mp1p in P. marneffei cells in yeast phase cultured at 37°C by two Mp1p antigen ELISAs; (C, D): evaluation of the specificity by detection of Mp1p in kinds of fungal cultured filtrates by MAb-MAb Mp1p ELISA (C) and PAbs-MAb Mp1p ELISA (D). The positive OD₄₅₀ values are restricted to cultured filtrate of P. marneffei in yeast phase, and are higher when cultured at 37°C.