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. 2011 Dec 21;6(12):e28835. doi: 10.1371/journal.pone.0028835

Figure 4. Increased lifespan in C. elegans by Icariside II treatment is dependent on the insulin/IGF-1 signaling.

Figure 4

A. Survival curves of N2, daf-2(e1370), and daf-16(mu86) hermaphrodites treated with DMSO control or 20 µM icariside II are shown. Icariside II treatment does not increase the life span in daf-2(e1370) and daf-16(mu86) mutant but extends the life span in N2. This is the representative of 3 independent experiments with similar results. Detailed parameters are presented in Table S2. B. Icariside II increases the mRNA expression of FOXO/DAF-16 targets hsp12.3 and sod3 significantly. Shown is the representative of three independent experiments with similar results. Error bars indicate SEM among three replicates in qRT-PCR; t-test, ***P<0.001. C. Representative images of the whole-animal of SOD-3::GFP transgenic strain CF1553 treated with DMSO or 20 uM icariside II from day 1 in adulthood to day 4 are shown. Icariside II increased the GFP fluorescence significantly compared to DMSO control. D. Western blot analysis shows an increase of GFP induced by 20 uM Icariside II treatment in SOD-3::GFP transgenic strain CF1553. Picture shown is the representative of three independent experiments with similar results. Levels shown are the average relative density level in 3 experiments; error bars indicate SEM among the density level of three experiments; t-test, ** P<0.01. E. Effect of icariside II is HSF-1 dependent. Survival curves of hsf-1 (sy441) treated with DMSO control and 20 uM icariside II are shown. Icariside II did not increase the lifespan in hsf-1 (sy441) mutant.