Table 2. Amniotic Fluid and Fetal Cultures.
Group | AF | Membrane | Fetal Meninges | Fetal Lung | Fetal CSF | Fetal blood | |
Inoculation site | Fundus | ||||||
Saline 1 | None | None | None | None | None | None | None |
Saline 2 | None* | None | None | None | None | None | None |
Saline 3 | None | None | None | None | None | None | None |
Saline 4 | None* | None | None | None | None | None | None |
Saline 5 | None | None | None | None | None | None | None |
GBS 1 | None* | None | None | None | None | None | None |
GBS 2 | None | None | None | None | None | None | None |
GBS 3 | None | None | None | None | None | None | None |
GBS 4 | None | 1,000 GBS, <100 S. aureus | None | None | None | None | None |
GBS 5 | None | 100 GBS | None | None* | None* | None | None |
Above values represent CFU/ml.
*A suspected contaminant (coagulase negative Staphylococcus, S. viridians) was noted in one AF culture of two saline controls and one GBS animal with all subsequent cultures being negative. Multiple suspected contaminants were noted in fetal tissue cultures from GBS #5 collected at the time of necropsy by an ill pathologist, who was coughing and wearing a mask (e.g. Streptococcus gordonii, Neisseria species).
AF, amniotic fluid; CSF, cerebrospinal fluid.