Table 1. Percentage of neurons expressing GFRα1-, GFRα2-, GFRα3- and TRPV1- immunoreactivity in DRG (in vivo) and 1 d after culturing (in vitro).

	GFRα1	GFRα2	GFRα3	TRPV1	IB4
in vivo (%)	33.5±1.9	33.3±2.6	24.8±0.8	30.3±1.5	28.3±0.8
in vitro (%)(24 h)	15.8±1.0*	2.1±0.6%*	43.9±2.9*	36.3±0.6*	43.6±1.4*

In vivo, the percentage of neurons expressing GFRα1-3 was similar. In vitro, GFRα2-positive cells were rarely seen 1 d after plating. The percentage of GFRα1-positive cells was decreased whereas the percentage of GFRα3-positive cells was increased. A small increase in the percentage of TRPV1-positive and IB₄-binding neurons also occurred (*p<0.05, t-test).