Table 2. Sensory neuron survival in vitro.
Control | NGF | GDNF | Artemin | NRTN | |
6 h (%) | 100 | 100 | 100 | 100 | 100 |
1 d (%) | 85.31±0.69 | 96.24±1.13* | 92.38±2.06* | 94.12±2.09* | 81.95±5.23 |
4 d (%) | 73.22±3.27 | 89.53±1.64* | 87.71±1.80* | 88.48±1.94* | 76.16±4.43 |
The number of NeuN-positive neurons 6 h after plating was designated as 100%. Without growth factors (Control), approximately 15% of neurons died 1 d after plating and 27% died by 4 d. Application of NGF, GDNF and ARTN significantly increased neuron survival although NRTN was ineffective.
*p<0.05; Two way ANOVA, Dunnett's post-hoc test.