Table 5. Primers (sequences and melting temperatures) used for real-time RT-PCR assays.

Gene	Forward Primer (5′–3′)	Tm(°C)	Reverse Primer (5′–3′)	Tm(°C)
GAPDH	ATGTGTCCGTCGTGGATCTGA	58.1	ATGCCTGCTTCACCACCTTCTT	58.6
GFRα1	GTGTGCAGATGCTGTGGACTAG	57.6	TTCAGTGCTTCACACGCACTTG	58.5
GFRα2	TGACGGAGGGTGAGGAGTTCT	59.1	GAGAGGCGGGAGGTCACAG	58.7
GFRα3	CTTGGTGACTACGAGTTGGATGTC	57.7	AGATTCATTTTCCAGGGTTTGC	54
Runx1	TTTCAAGGTACTCCTGCCTGA	55.2	CAGTGAGAAGGACCAGAGACT	55.3