Figure 6. Comet assay analysis of DNA damage and DNA damage repair under SMG or 1G.
(A) Evaluation of DNA damage by the alkaline comet assay in mES cells after two days incubation under SMG or 1G. (B) Kinetics of comet tail moment in mES cells irradiated with 8 Gy of gamma-ray and incubated in 1G or in SMG evaluated by neutral comet assay. Time points were 0 hr, 1 hr, 2 hr. On the right representative comet assay results were shown, and on the left quantitative comparison of comet tail moments between the 1G group and the SMG group. At least 50 cells of each time point was scored for comet tail moment. The data represents mean ± SD of three independent experiments. Student's t test, *p<0.05.