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. 2011 Dec 21;6(12):e29191. doi: 10.1371/journal.pone.0029191

Figure 2. Isolation of the membrane fraction from E. coli cells.

Figure 2

In this case extracts of E. coli cells overexpressing either the Mistic fusion of the protein NapC or the protein P450. cl stands for “cleared lysate” corresponding to the supernatant recovered after centrifugation, at 20,000 g, of the cell lysate. hs and M stand for “high-speed supernatant” and “membrane fraction”, respectively, corresponding to the supernatant and the resuspended pellet recovered after ultra-centrifugation, at 100,000 g, of the “cleared lysate”. Arrows point out the different target proteins and the endogenous E. coli biotinylated protein BCCP. The membrane was probed with the Strep-Tactin HRP conjugate (IBA).