| Does simulation of herbivory by mechanical wounding elicit an activated defence? |
LC/MS and GC/MS based metabolomics to study metabolic responses in mechanically wounded tissue. Resulted grazer deterrence assessed by Idotea baltica bioassays. |
| Follow-up question: Are up-regulated metabolites in the activated defence produced de-novo from arachidonic acid? |
LC/MS analysis of labelling pattern following administration of deuterated arachidonic acid to mechanically wounded tissue. |
| Does mechanical wounding cause a similar metabolic response as natural grazing? |
Exposure of algae to 1 and 2 h intense grazing by Idotea baltica followed by targeted analysis of the metabolites significantly up-and down regulated in response to mechanical wounding. |
| Can a defence be induced by signals from attacked tissue? |
LC/MS and GC/MS based metabolomics to study metabolic responses in intact tissue exposed to extracts from mechanically wounded algae. Resulted grazer deterrence assessed by Idotea baltica bioassays. |
| Does local mechanical wounding lead to an activated defence reaction in neighbouring tissue? |
Local mechanical wounding of tissue and extraction of intact neighbouring tissue for targeted analysis of the metabolites significantly up-and down regulated in response to mechanical wounding. |
