Table 3. Metabolic response of Gracilaria vermiculophylla analysed by GC/MS.

	Label	Retention time (min)	Base peak1	Suggested structure2	Peak area relative to controls
A	A	6.28	131		1.4±0.1
	B	9.25	129		12.2±3.1
	C	13.15	243		14.7±3.0
	D	15.22	339	oleic acid	2.2±0.5
	E	17.46	239		9.9±1.9
	F	17.69	287	7,8-di-HETE	62.1±22.7
	G	18.04	436	15-keto-PGE2	2.3±0.6
	H	18.27	225	PGE2	2.2±0.6
B	1	6.13	174	n-butylamine	1.8±0.1
	2	6.17	116		1.5±0.1
	3	7.03	210		1.1±0.0
	4	7.50	117		1.1±0.0
	5	7.73	299	phosphoric acid	1.4±0.1
	6	8.08	174	glycine	1.7±0.2
	7	8.52	204	serine	1.9±0.2
	8	8.73	218	threonine	1.2±0.0
	9	9.35	350		1.2±0.1
	10	9.52	234	saccharide	1.4±0.1
	11	9.62	233	malic acid	1.5±0.1
	12	9.97	156	pyroglutamic acid	1.4±0.1
	13	10.19	292	rythroric acid	1.2±0.1
	14	10.35	198		1.7±0.1
	15	10.67	142		1.5±0.1
	16	10.70	246	glutamine	1.7±0.1
	17	10.90	315		1.7±0.4
	18	11.75	299	glycerophosphate	1.8±0.2
	19	11.85	319		1.8±0.1
	20	11.89	156		1.7±0.2
	21	11.95	392		1.6±0.1
	22	12.17	273	citric acid	1.2±0.0
	23	12.19	142	ornithine	1.2±0.0
	24	12.24	157		1.5±0.0
	25	12.30	287	methyl citric acid	1.4±0.1
	26	12.40	173		1.6±0.1
	27	12.47	117	myristic acid	1.3±0.0
	28	12.70	319	monosaccharide	1.8±0.1
	29	12.90	260		1.4±0.1
	30	13.05	218		1.6±0.2
	31	13.27	319	monosaccharide	1.4±0.1
	32	13.70	319	monosaccharide	1.8±0.1
	33	13.79	313	palmitic acid	1.3±0.0
	34	14.02	217	myo-inositol	1.2±0.1
	35	14.59	319	monosaccharide	2.3±0.2
	36	15.74	117	arachidonic acid	1.6±0.1
	37	16.03	527		1.5±0.1
	38	16.86	371	monopalmitin glycerol	1.5±0.2
	39	16.94	283		1.1±0.1
	40	17.18	572		2.0±0.2
	41	17.26	557		1.6±0.2
	42	17.64	361	disaccharide	5.9±0.8
	43	19.83	204	saccharide	2.5±0.2
	44	20.36	129	cholesterol	1.4±0.1

A: metabolites significantly up-regulated followed by mechanical simulation of herbivory. B: metabolites significantly up-regulated followed by exposure to extracts from previously mechanically wounded algae. Metabolic responses are presented as peak areas normalised to corresponding controls (means±SE, n = 8 (A), or 7 (B)). Suggested structures are from comparisons of MS spectral data with spectra in the NIST library. Metabolites with confirmed structure by comparisons to known standards in bold.

¹The most intense ion is given (the ion m/z = 73 in not considered in this listing).

²Library suggestion are only included if the match and reverse match were more than 790.