Table 3. Metabolic response of Gracilaria vermiculophylla analysed by GC/MS.
Label | Retention time (min) | Base peak1 | Suggested structure2 | Peak area relative to controls | |
A | A | 6.28 | 131 | 1.4±0.1 | |
B | 9.25 | 129 | 12.2±3.1 | ||
C | 13.15 | 243 | 14.7±3.0 | ||
D | 15.22 | 339 | oleic acid | 2.2±0.5 | |
E | 17.46 | 239 | 9.9±1.9 | ||
F | 17.69 | 287 | 7,8-di-HETE | 62.1±22.7 | |
G | 18.04 | 436 | 15-keto-PGE2 | 2.3±0.6 | |
H | 18.27 | 225 | PGE2 | 2.2±0.6 | |
B | 1 | 6.13 | 174 | n-butylamine | 1.8±0.1 |
2 | 6.17 | 116 | 1.5±0.1 | ||
3 | 7.03 | 210 | 1.1±0.0 | ||
4 | 7.50 | 117 | 1.1±0.0 | ||
5 | 7.73 | 299 | phosphoric acid | 1.4±0.1 | |
6 | 8.08 | 174 | glycine | 1.7±0.2 | |
7 | 8.52 | 204 | serine | 1.9±0.2 | |
8 | 8.73 | 218 | threonine | 1.2±0.0 | |
9 | 9.35 | 350 | 1.2±0.1 | ||
10 | 9.52 | 234 | saccharide | 1.4±0.1 | |
11 | 9.62 | 233 | malic acid | 1.5±0.1 | |
12 | 9.97 | 156 | pyroglutamic acid | 1.4±0.1 | |
13 | 10.19 | 292 | rythroric acid | 1.2±0.1 | |
14 | 10.35 | 198 | 1.7±0.1 | ||
15 | 10.67 | 142 | 1.5±0.1 | ||
16 | 10.70 | 246 | glutamine | 1.7±0.1 | |
17 | 10.90 | 315 | 1.7±0.4 | ||
18 | 11.75 | 299 | glycerophosphate | 1.8±0.2 | |
19 | 11.85 | 319 | 1.8±0.1 | ||
20 | 11.89 | 156 | 1.7±0.2 | ||
21 | 11.95 | 392 | 1.6±0.1 | ||
22 | 12.17 | 273 | citric acid | 1.2±0.0 | |
23 | 12.19 | 142 | ornithine | 1.2±0.0 | |
24 | 12.24 | 157 | 1.5±0.0 | ||
25 | 12.30 | 287 | methyl citric acid | 1.4±0.1 | |
26 | 12.40 | 173 | 1.6±0.1 | ||
27 | 12.47 | 117 | myristic acid | 1.3±0.0 | |
28 | 12.70 | 319 | monosaccharide | 1.8±0.1 | |
29 | 12.90 | 260 | 1.4±0.1 | ||
30 | 13.05 | 218 | 1.6±0.2 | ||
31 | 13.27 | 319 | monosaccharide | 1.4±0.1 | |
32 | 13.70 | 319 | monosaccharide | 1.8±0.1 | |
33 | 13.79 | 313 | palmitic acid | 1.3±0.0 | |
34 | 14.02 | 217 | myo-inositol | 1.2±0.1 | |
35 | 14.59 | 319 | monosaccharide | 2.3±0.2 | |
36 | 15.74 | 117 | arachidonic acid | 1.6±0.1 | |
37 | 16.03 | 527 | 1.5±0.1 | ||
38 | 16.86 | 371 | monopalmitin glycerol | 1.5±0.2 | |
39 | 16.94 | 283 | 1.1±0.1 | ||
40 | 17.18 | 572 | 2.0±0.2 | ||
41 | 17.26 | 557 | 1.6±0.2 | ||
42 | 17.64 | 361 | disaccharide | 5.9±0.8 | |
43 | 19.83 | 204 | saccharide | 2.5±0.2 | |
44 | 20.36 | 129 | cholesterol | 1.4±0.1 |
A: metabolites significantly up-regulated followed by mechanical simulation of herbivory. B: metabolites significantly up-regulated followed by exposure to extracts from previously mechanically wounded algae. Metabolic responses are presented as peak areas normalised to corresponding controls (means±SE, n = 8 (A), or 7 (B)). Suggested structures are from comparisons of MS spectral data with spectra in the NIST library. Metabolites with confirmed structure by comparisons to known standards in bold.
The most intense ion is given (the ion m/z = 73 in not considered in this listing).
Library suggestion are only included if the match and reverse match were more than 790.