Figure 2. Apigenin induces ROS accumulation in HeLa cells.

A, HeLa cells were treated with apigenin (15 µM) alone or together with paclitaxel (4 nM), for indicated periods. DHE (5 µM) was added into cell cultures 30 minutes before the treatment. DHE staining in cells were detected and analyzed by Guava EasyCyte™ System. DHE staining in untreated cells were used as the negative control. B, CM-H₂DCFDA (5 µM) staining was performed and analyzed as described in A.