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. 2011 Dec 21;6(12):e29390. doi: 10.1371/journal.pone.0029390

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Perrin-Tricaud et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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The enzymatic activity is expressed relatively to the activity of wildtype GLT25D1. Values show the means and S.E.M. of at least four independent assays. The expression of the mutant constructs CEECAM1-CterB[S461D] and CEECAM1-CterB[S461D][RMQV] is confirmed by Western blotting performed in Sf9 cell lysate using an anti-Flag antibody. RMQV stands for the substitution Q471R-V472M-N473Q-P474V.