Table 1. Kinetic parameters for chimeric constructs CEECAM1-MidC and CEECAM1-CterC.
UDP-Gal | Collagen | |||
Enzyme | Km (µM) | Vmax (pmol min−1) | Km (µg µl−1) | Vmax (CPM min−1) |
GLT25D1 | 29.91±2.56 | 12.45±1.09 | 5.7±0.9 | 235.13±25.66 |
CEECAM1-MidC | 20.31±1.63 | 6.86±0.45 | 5.04±1.12 | 108.53±29.95 |
CEECAM1-CterC | 74.29±5.56 | 11.2±1.4 | 13.4±1.75 | 259.27±63.48 |
Values represent the average ± S.E.M. of three independent experiments.