Table 3. Sense strand oligonucleotides used for site-directed mutagenesis.

Plasmid template	Mutations	Oligonucleotide sequence (5′-3′)
GLT25D1	D166A-D168A	CATCCTGTTTGTAGCGGCCGCCAACCTGATCCTCAACCC
GLT25D1	P292N	GGAGTACGGATTCTTGAACGTTCCATTGCGCGCCCACAGC
GLT25D1	D336S	CCCACCAAGACACCGAGCAAGATGGGCTTCG
GLT25D1	D461A-D463A	GGAGGGCCTGGCCTGGGCCCTCATCTATGTGG
GLT25D1	D585A-D587A	CGTCAAGACCGCCTGGGCCCGCGCCAAGTCC
CEECAM1-CterB	S461D	GGAGGCAGAGAAACTGGATTGGGACCTGATCTACC
CEECAM1-CterB[S461D]	Q471R-V472M-N473Q-P474V	GATCTACCTCGGACGGAAGCGCATGCAGGTTGAGAAGGAGACGGCCGTGG

Sequences show the sense strand oligonucleotides of each complementary pair of primers used for site-directed mutagenesis. Underlined bases mark the introduced mutations.