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. 2011 Dec 21;6(12):e29216. doi: 10.1371/journal.pone.0029216

Figure 1. Effect of tunicamycin (TM) on the growth and budding of P. brasiliensis yeast cells.

Figure 1

MTT assay of yeast cells cultivated in YPD medium in the absence (white bars) or presence (black bars) of 15 µg/mL TM (A). Number of cells with buds in YPD medium in the absence (white bar, CTL) or presence (black bar, TM) of 15 µg/mL TM for 72 h at 37°C, and analyzed by optical microscopy and counted using neubauer chamber (B). Western blotting with ECL glycoprotein detection of the crude extract fully glycosylated (PbAg), crude extract underglycosylated (PbAgTM) and crude extract fully glycosylated treated with N-glycanase PNGase F (PbAg + PNGase F) (C). Data are representative of 3 independent assays. * p<0.05 for comparison between CTL and TM.