Figure 3. Effect of tunicamycin (TM) on the distribution of paracoccin in P. brasiliensis yeasts.

Fluorescence labeling with anti-paracoccin IgY was evenly distributed over the yeast surface. In some budding regions, a more intense labeling was detected for untreated P. brasiliensis yeast cells (B, C, I and J) compared with those treated with TM (E, F, M and N), which were more centrally labeled. Panels H and L show fluorescence labeling chitin, while panels B, E, I and M show fluorescence labeling paracoccin. C is the merge of A and B; F is the merge of D and E; J is the merge of G, H and I; and N is the merge of K, L and M.