Table 2. Maintenance of native pRM in shuttle vector-transformed R. monacensis.
| Rickettsial Culture | Relative Copy Number of pRM (hsp2/gltA) | Relative Copy Number of Shuttle Vector (gfpuv/gltA) |
| R. monacensis | 2.7 | NA |
| R. monacensis pRAM32dRGA transformant subculture 1 | 4.2 | 3.2 |
| R. monacensis pRAM18dRGA transformant subculture 1 | 3.1 | 4.8 |
| R. monacensis pRAM18dRGA transformant subculture 8 | 2.9 | 5.4 |
| R. monacensis pRAM18dRGA transformant subculture 15 | 3.1 | 5.5 |
| R. monacensis pRAM18dRGA transformant subculture 15 (No Rif for 5 subcultures) | 2.5 | 3.6 |