Table 2. Number of Pseudonocardia strains found per nest of Trachymyrmex septentrionalis. The culture-dependent counts are presented as the number of P. cf. carboxydivorans isolates of morphotype A–D found in a particular nest. The culture-independent counts list the total number of Pseudonocardia 454-sequences found per ant caste and the number of different Pseudonocardia strains among these sequences. Empty cells indicate absence of Pseudonocardia, and “na” indicates that a particular sample type (e.g., reproductive ant) was not found in a nest at the time of collection. Because of an oversight, nests JLM090322-04 and JLM090630-02B were only screened in the culture-independent screen. In our custom Pseudonocardia BLAST, the majority of the sequences were identified as P. cf. carboxydivorans (i.e., sequence accession FJ490549 was hit in the BLAST of every ant sample; see also BLAST-results identifying clade-3 Pseudonocardia in Supplementary Table S5).
| Culture-Dependent Screen | Culture-Independent Screen | |||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Nest ID (Abbreviated nest ID) | P. cf. carboxydivorans Cultured Morphotypes | |||||||||||||||
| Month | A | B | C | D | # of Pseudonocardia 454-sequences | Distinct # of Pseudonocardia strains per nest | ||||||||||
| garden worker | outside worker | male | female | outside worker | garden worker | outside worker | female | garden worker | outside worker | garden worker | outside worker | female | male | |||
| JLM090131-01 | Jan | 2 | na | na | na | na | na | na | na | na | ||||||
| JLM090131-03 | Jan | 1 | na | na | na | na | na | na | na | na | ||||||
| JLM090207-01 (J0201) | Feb | na | na | na | 30 | 29 | na | na | 1 | |||||||
| JLM090322-01 (J8-12) | Mar | na | na | na | 74 | 16 | na | na | 2 | |||||||
| JLM090322-2A (J0302A) | Mar | na | na | na | 1831 | 47 | na | na | 6 | |||||||
| JLM090322-2B (J0302B) | Mar | na | na | na | 124 | 67 | na | na | 4 | |||||||
| JLM090322-03 (J0303) | Mar | na | na | na | 157 | 36 | na | na | 4 | |||||||
| JLM090322-04 (J0304) | Mar | na | na | na | na | na | na | na | na | na | na | 20 | 11 | na | na | <1?h=-12pt?>2 |
| JLM090405-01 (J0401) | Apr | 11 | 6 | na | na | na | 59 | 100 | na | na | <1?h=-12pt?>2 | |||||
| JLM090405-02 (J0402) | Apr | 3 | na | na | na | 66 | 108 | na | na | 1 | ||||||
| JLM090405-03 (J0403) | Apr | 7 | 3 | na | na | na | 132 | 182 | na | na | 1 | |||||
| JLM090405-04 (J0404) | Apr | 3 | 1 | na | na | na | 1 | 55 | 128 | na | na | 3 | ||||
| JLM090528-01 (J0501) | May | 1 | 1 | na | na | 2 | 3 | na | 1 | 46 | 96 | na | na | 2 | ||
| JLM090528-02 (J0502) | May | 7 | 7 | na | na | na | 46 | 59 | na | na | <1?h=-12pt?>1 | |||||
| JLM090528-03 (J0503) | May | na | na | na | 233 | 204 | na | na | 3 | |||||||
| JLM090528-04 (J0504) | May | 3 | 9 | na | na | na | 2 | 218 | 144 | na | na | 5 | ||||
| JLM090627-01 (J0601) | Jun | 10 | na | 436 | 357 | na | na | <1?h=-12pt?>3 | ||||||||
| JLM090627-02 (J0602) | Jun | 55 | na | na | 52 | 299 | na | na | 1 | |||||||
| JLM090627-03 (J0603) | Jun | 13 | na | na | 124 | 56 | na | na | 2 | |||||||
| JLM090630-02 (J1-J7) | Jun | 6 | 25 | 35 | 24 | 273 | 125 | 3081 | 1806 | 7 | ||||||
| JLM090630-02B (J0630-02) | Jun | na | na | na | na | na | na | na | na | na | na | na | na | 5440 | 286 | 3 |
| JLM090630-03 (J0630-03) | Jun | 10 | 9 | na | 17 | 72 | 93 | 521 | na | 2 | ||||||
| RS090713-01 | Jul | 3 | 3 | na | na | na | na | na | na | na | ||||||
| JLM090713-01 (J0701) | Jul | 25 | 28 | na | 27 | 83 | 84 | 729 | na | 2 | ||||||
| JLM090713-02 (J0702) | Jul | 8 | 33 | na | na | 152 | 50 | na | na | <1?h=-12pt?>2 | ||||||
| JLM090713-04 (J0704) | Jul | na | 4 | 56 | 233 | 153 | 1513 | na | <1?h=-12pt?>7 | |||||||
| JLM090830-01 (J0801) | Aug | 29 | 47 | na | 22 | 184 | 171 | 110 | na | <1?h=-12pt?>2 | ||||||
| JLM090830-02 (J0802) | Aug | na | na | 5 | 2476 | 167 | na | na | 2 | |||||||
| JLM090830-03 (J0803) | Aug | 6 | na | 82 | 181 | 681 | na | 6 | ||||||||
| Average | 290.32 | 118.52 | 1725 | 1046 | 2.92 | |||||||||||