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. Author manuscript; available in PMC: 2013 Jan 1.
Published in final edited form as: Mol Cell Neurosci. 2011 Sep 10;49(1):32–43. doi: 10.1016/j.mcn.2011.08.011

Figure 8. Ventral r1 GABAergic identity is PITX2-independent.

Figure 8

Sagittal E18.5 Nestin-Cre;Pitx2flox/+ and Nestin-Cre;Pitx2flox/tlz littermate brains processed for single in situ hybridization for Pitx2, Gad1, or Vglut2 or immunohistochemistry with antibodies against 5-HT. (A) Cartoon of a sagittal section showing the orientation of panels B–G. (B–G) Merged images of neighboring sections processed for in situ hybridization for Pitx2 and either Gad1 (B, E), Vglut2 (C, F), or 5-HT (D, G) immunohistochemistry and pseudocolored. Scale bar in B is 200 µm and applies to panels B–G.