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. Author manuscript; available in PMC: 2013 Jan 1.
Published in final edited form as: Cell Immunol. 2011 Oct 25;272(2):220–229. doi: 10.1016/j.cellimm.2011.10.008

Fig. 1.

Fig. 1

Targeting TLR4 and Myd88 using RNA interference. Messenger RNA was isolated from 4T1 cells 24 hours after treatment with different siRNA specific for TLR4 (A) or Myd88 (B), and screened by QRT-PCR for decreased gene expression using gapdh as the reference gene, and lacZ siRNA treated cells as the control. 4T1 were transfected with a shRNA expression vector specific for TLR4 (C) or Myd88 (D) and screened two weeks after G418 selection by QRT-PCR for decreased gene expression using gapdh as the reference gene, and cells transfected with a lacZ specific shRNA expression vector as the control. All data represent the average and standard error of 3 separate experiments.