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. Author manuscript; available in PMC: 2013 Jan 1.
Published in final edited form as: J Immunol. 2011 Dec 2;188(1):85–102. doi: 10.4049/jimmunol.1003804

FIGURE 11.

FIGURE 11

Respiratory burst responses of ATRA-induced EPRO cells treated with LPDS vs. ATRA-induced EPRO-ic/ic cells transduced with full-length or truncated variants of Lbr. A, EPRO-+/+ cells were cultured in either regular medium with ATRA, medium with LPDS plus ATRA, or pre-cultured for 3 days in medium with LPDS and then induced in medium with LPDS plus ATRA. Respiratory burst responses were measured after 5 days of induction and stimulation with 3.2μM phorbol myristate acetate (PMA) using luminol-enhanced chemiluminescence at one minute intervals. B, EPRO-+/+ cells cultured in regular medium, medium with LPDS or medium with lovastatin during ATRA induction were tested for respiratory burst responses caused by opsonized zymosan (OZ). C, Respiratory burst responses to PMA after 5 days of ATRA induction in regular medium were measured from EPRO cells of the indicated genotypes. Values of relative light units in each graph are averages ± SD from triplicate assays performed in parallel.