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. Author manuscript; available in PMC: 2013 Jan 1.
Published in final edited form as: J Immunol. 2011 Dec 2;188(1):85–102. doi: 10.4049/jimmunol.1003804

FIGURE 3.

FIGURE 3

Lipid production in oleic acid-induced cells and cholesterol biosynthesis is disrupted by loss of Lbr expression. (A-C) EML, EPRO and ATRA-induced EPRO cells from each genotype were cultured in medium that contained 10% oleic acid for 3 days, then fixed with 4% paraformaldehyde and stained with Nile Red. A, Cells stained for Nile red were photographed under fluorescence to visualize lipids (original magnification, x 400). The numbers of yellow droplets indicate the amount of lipids synthesized in each cell. Graphed are the percentage of +/+, +/ic and ic/ic cells positively stained for lipids (B), and the average number of individual droplets that could be counted per cell (C). Data are the average counts of approximately 100 analyzed cells from each induction ± SD. D, GC/MS analysis of EML cells of each genotype cultured in regular medium (upper panels) or LPDS medium (lower panels) reveals changes in sterol levels. Shown are chromatographs that reveal the relative abundance of extracted sterols with respect to stigmasterol loaded as an internal control. Labeled peaks correspond to cholesterol (peak 1, confirmed by analyses of cholesterol alone), two precursors (peaks 2 and 3), and stigmasterol (peak 4).