Fig 1. RIP1 protein structure and expression.
A, A diagram of the human RIP1 protein is shown, which consists of an amino terminal serine/threonine kinase domain (KD) and carboxy terminal death domain (DD). The intermediate domain between the KD and DD contains a RIP homotypic interaction motif (RHIM), a caspase 8 cleavage site (D324), and a ubiquitinylation site (K377). B, Western blotting analysis shows the expression of the RIP1 protein in wild type E17.5 mouse embryos, adult mouse tissues, and purified lymphocytes. The RIP1−/− mutant embryo was used as a control.