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. 2011 Dec 22;2:259. doi: 10.3389/fmicb.2011.00259

Figure 2.

Figure 2

(A,B) Systemic infection of Chenopodium quinoa by BVQ RNA transcripts. (A) Upper non-inoculated leaves show chlorosis and necrosis and (B) local lesions induced on inoculated leaves. (C,D) Single confocal microscope sections showing nuclear and nucleolar accumulation of YFP-TGB1 expressed from the PMTV reporter clone. [(C) left panel) YFP-TGB1 and (D): YFP-del84-TGB1. [(C,D) right panels], co-expression of the respective proteins with monomeric red fluorescent protein-labeled Arabidopsis fibrillarin 2 (magenta) to distinguish the nucleolus. Scale bar = 5 μm. (E) Model of PMTV RNA movement. (1) Viral RNP complexes are transported intra-cellularly by TGB2 and TGB3 on the endoplasmic reticulum-actin network to the PD. (2) TGB1 enters the nucleolus where it either acquires a host factor or is modified in some way, or acts to modify the host to facilitate long-distance movement of RNP or (3), tipped virions in the vasculature. (4) Virions that do not contain CP-RT or that contain mutant CP-RT that does not interact with TGB1 are not competent for long-distance movement.