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. Author manuscript; available in PMC: 2011 Dec 22.
Published in final edited form as: Nat Med. 2011 Aug 21;17(9):1076–1085. doi: 10.1038/nm.2439

Figure 3.

Figure 3

Mitochondrial OXPHOS function and oxidative stress in cardiac muscle of wild-type and Atg/KO mice. (a,b) Oxygen consumption, an indicator for mitochondrial respiration, in Atg/KO cardiac homogenates of 4-week-old (a) and 8-week-old (b) male mice in the presence of glucose (n = 6). (c) Triglyceride (TG) content in cardiac muscle of wild-type and Atg/KO mice. (d,e) Oxygen flux of mitochondria isolated from cardiac tissue of 8- to 9-week-old male wild-type and Atg/KO mice. ADP-driven (state 3) and uncoupled (state U) oxygen flow was measured in the presence of pyruvate (d) and palmitoyl-CoA (e) in subsarcolemmal (SS) and in intramyofibrillar (IMF) mitochondria (n = 6). (f) Western blotting analysis of mitochondrial respiratory chain proteins NDUFA9 of complex I and SDHA of complex II in mitochondrial preparations of Atg/KO mice and wild-type mice. MTCO1, a marker of complex IV, served as loading control. (g) Mitochondrial membrane potential (tetramethyl-rhodaminemethylester perchlorate (TMRM) staining) in isolated cardiac mitochondria of 8- to 9-week-old female Atg/KO compared to wild-type mice (n = 4). (h) Relative concentrations of non-oxidized (free) thiol groups in isolated mitochondria of 8- to 9-week-old female Atg/KO mice compared to wild-type mice (n = 4). Error bars are means ± s.d. *P < 0.05, **P < 0.01 and ***P < 0.001.