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. Author manuscript; available in PMC: 2012 Dec 22.
Published in final edited form as: Neuron. 2011 Dec 22;72(6):964–976. doi: 10.1016/j.neuron.2011.12.003

Figure 2.

Figure 2

Molecular Cloning of insomniac

(A) Schematic of CG14795 and CG32810 loci and inc mutants. Exons are shown as thicker bars, introns by connecting lines. Arrowheads below wild-type locus denote location of primers used for PCR amplification and 5' RACE. (B) Sequence of CG14795 first exon (cyan), promoter region, and portion of CG32810 first exon (magenta). Uppercase, transcribed sequence; arrows indicate putative transcription initiation sites of longest 5' RACE products; start codons are underlined. Sequence removed by the inc1 deletion is shaded. Arrowhead indicates PBac{WH}CG32810f00285 insertion site in the inc2 mutant. (C) PCR amplification of wild-type and inc1 genomic DNA with primers shown in (A). (D) 5' RACE analysis of CG32810 and CG14795. (E) Northern analysis of CG32810 and CG14795 transcripts. (F) Western blot analysis for Insomniac protein. See also Figures S1 and S4.