Skip to main content
. Author manuscript; available in PMC: 2012 Dec 23.
Published in final edited form as: Immunity. 2011 Dec 23;35(6):919–931. doi: 10.1016/j.immuni.2011.11.012

Figure 6. Effect of Modulating the Levels of IFN-γand IL-21 during the Course of In Vitro Th1 Differentiation.

Figure 6

(A–F) Sorted naïve CD4+ T cells were cultured under IL-12 conditions for 3 to 5 days. Combinations of IFN-γ, anti- IFN-γ antibody, IL-21, or soluble IL-21 receptor-Fc were added as indicated to enhance or deplete the levels of IFN-γ or IL-21 in the culture.

(A, B, D, E) Levels of IL-21 and IFN-γ (A, D), and CXCR5 and PD-1 (B, E) on CD4+ cells were determined by FACS at day 3 and/or 5.

(C, F) Levels of Bcl6 and T-bet on CD4+ cells were determined by FACS and by Western blotting at day 5.

Representative data of three independent experiments are shown (mean ± SD). *, p<0.05, **, p<0.01.