Figure 3.

Effects of ESP (epithiospecifier protein) and NSP (nitrile-specifier protein) on the hydrolysis of allylglucosinolate in vitro.

Shown are total ion current traces of GC-MS chromatograms of enzyme assays after extraction with dichloromethane (A–C) and chemical structures of allylglucosinolate hydrolysis products (D). Allylglucosinolate (2 mM) was incubated with myrosinase in the presence of purified ESP (A), NSP1 (B), or without addition of specifier proteins (C) in 50 mM MES buffer, pH 6.0. IS, internal standard; peak numbers refer to the structure numbers in D. 1, simple nitrile; 2, isothiocyanate; 3, epithionitrile.