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. Author manuscript; available in PMC: 2012 Dec 1.
Published in final edited form as: J Immunol. 2011 Nov 7;187(11):5515–5519. doi: 10.4049/jimmunol.1102497

FIGURE 4.

FIGURE 4

CD28 requires both ligand-binding sites within a dimer to be efficiently recruited to the immunological synapse. CD28KO T cells were transfected with WT-VN and WT-VC (WT/WT), with WT-VN and Y104A-VC (WT/Mut), or with Y104A-VN and Y104A-VC (Mut/Mut) and T cell:APC conjugates were analyzed for localization of CD28 dimers by fluorescence of reassembled VN/VC fragments. A, Representative images. B, The efficiency of CD28 recruitment to the immunological synapse (ratio of CD28 IS/back), population medians and p values (Mann-Whitney) are shown.