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. Author manuscript; available in PMC: 2011 Dec 22.
Published in final edited form as: Chembiochem. 2010 Sep 3;11(13):1889–1895. doi: 10.1002/cbic.201000358

Table 1.

Comparison of the TROL and ThT protocols.

Tryptophanol (TROL) assay Thioflavin T (ThT) assay
1) add 10 μL of amyloid solution to 96-well plate[a] 1) add 10 μL of amyloid solution to 96-well plate[a]
2) add 100 μL TROL (10 μM) per well (50 mM glycine, pH 8.2, 0.01 % DMSO) 2) add 200 μL filtered (0.22 μm) ThT (5 μM) per well (50 mM glycine, pH 8.2)
3) mix by pipetting, incubate 5 min (37 °C) and then cool 15 min (RT) 3) mix by pipetting and incubate 15 min (RT)
4) read fluorescence (λex = 280/λem = 355) 4) read fluorescence (λex = 446/λem = 490)
[a]

96-well black, opaque, flat-bottomed microplate.