TABLE 1.

Amplitudes and rate constants for amide H/D exchange of selected peptides (from Figure 5) in the presence of GSH and inhibitors 2, 3 and 4.

Peptide/Ligand	Afast	A1 (D)	k1 (min-1)	A2 (D)	k2 (min-1)
A 37-54 (16)
GSH	3.9	5.90 ± 0.15	0.060 ± 0.005	6.18 ± 0.26	≤ 1 × 10-4
2	9.4	3.54 ± 0.12	0.019 ± 0.003	3.09 ± 0.69	≤ 1 × 10-4
3	7.9	6.16 ± 0.11	0.027 ± 0.002	1.93 ± 0.18	≤ 1 × 10-4
4	3.5	7.34 ± 0.14	0.079 ± 0.005	5.19 ± 0.15	≤ 1 × 10-4
B 78-83 (4)
GSH	0.8	0.94 ± 0.02	0.0043 ± 0.0004	2.30 ± 0.13	≤ 1 × 10-4
2	0	2.28 ± 0.04	0.021 ± 0.001	1.69 ± 0.18	≤ 1 × 10-4
3	0	0.6 ± 0.2	0.61 ± 0.03	3.62 ± 0.02	≤ 4.80 × 10-4
4	0	0.59 ± 0.08	0.75 ± 0.02	3.35 ± 0.03	≤ 8.29 × 10-4
C 104-107 (3)
GSH	0	0.37 ± 0.05	0.036 ± 0.002	2.56 ± 0.05	≤ 4.90 × 10-4
2	0	3	≤ 1 × 10-4
3	0	3	≤ 1 × 10-4
4	0	3	≤ 1 × 10-4
D 124-129 (5)
GSH	0	0.99 ± 0.04	0.023 ± 0.002	4.00 ± 0.16	≤ 1 × 10-4
2	0.4	1.54 ± 0.03	0.018 ± 0.002	3.02 ± 0.18	≤ 1 × 10-4
3	0	5	≤ 1 × 10-4
4	0.3	1.84 ± 0.04	0.036 ± 0.003	2.92 ± 0.10	≤ 1 × 10-4
E 130-132 (2)
GSH	0.3	0.90 ± 0.02	0.045 ± 0.005	0.75 ± 0.05	≤ 1 × 10-4
2	0	0.78 ± 0.04	0.13 ± 0.02	1.21 ± 0.04	≤ 1 × 10-4
3	0.1	0.48 ± 0.02	0.017 ± 0.003	1.37 ± 0.10	≤ 1 × 10-4
4	0.1	0.85 ± 0.01	0.020 ± 0.001	0.95 ± 0.05	≤ 1 × 10-4
F 133-140 (6)
GSH	0	1.12 ± 0.03	0.0045 ± 0.0006	4.88 ± 0.03	≤ 1 × 10-4
2	0.4	3.50 ± 0.06	0.0079 ± 0.0006	2.12 ± 0.06	≤ 1 × 10-4
3	0.7	2.85 ± 0.06	0.0044 ± 0.0005	2.40 ± 0.06	≤ 1 × 10-4
4	0.4	1.25 ± 0.11	0.06 ± 0.01	4.28 ± 0.11	≤ 1.09 × 10-3