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. Author manuscript; available in PMC: 2012 Dec 22.
Published in final edited form as: Neuron. 2011 Dec 22;72(6):991–1000. doi: 10.1016/j.neuron.2011.11.014

Figure 6. Like WT, the charge-swapped double mutant D112R-R3D does not conduct Gu+.

Figure 6

Inside-out patches were initial recorded in symmetric 100 mM Gu+ pH 8 solution (black traces), and subsequently internally perfused with 0 mM Gu+ pH 6 solution. Mutant R3D displays an outward current in symmetric Gu solution (marked by an arrow), whereas WT and D112R-R3D do not. Relative maximal outward current reduction by 100 mM Gu pH 8 vs 0 mM Gu pH6 was 99.1 ± 0.7 % for WT, 52.2 ± 13.6 % for D112R-R3D, and 92.3 ± 4.7 % for D112R-R3D. Bar graphs display the mean outward current (measured at the end of the depolarizing voltage step). Data points are shown as circles connected by lines for each individual patch. Errors bars denote s.e.m.