Consensus domain structure of the NPH3/RPT2 (NRL) protein family.
(A) The DIa, DIb, DII, DIII, and DIV regions (black bars) represent areas of sequence conservation among members of the NPH3/RPT2 protein family. The BTB/POZ (red region) and coiled-coil (blue region) domains represent structurally conserved motifs. While not all members of the family contain all of these domains (see cladogram in Figure 4B), each domain is present in both NPH3 and RPT2 (Motchoulski and Liscum, 1999; Sakai et al., 2000). Inter-domain regions (grey) while not sequence conserved exhibit moderate structural similarities from member to member (see http://www.biosci.missouri.edu/liscum/nph3-rpt2figs.html).
(B) Cladogram of the NRL protein family based on amino acid sequence obtained from Arabidopsis genome annotation (TAIR8) at TAIR. NRL protein sequences were aligned by ClustalW and the resulting ClustalW alignment was used to generate a rooted phylogenetic tree (Cladogram). Corresponding AGI numbers for a given NRL member is given in parenthesis.