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. 2011 Dec 22;6(12):e29172. doi: 10.1371/journal.pone.0029172

Figure 1. The ind CRM contains binding sites that mediate repression in dorsal regions.

Figure 1

lacZ reporter expression was visualized within cellularized embryos (late stage 5) by in situ hybridization using a digoxigenin-labeled antisense lacZ riboprobe. In this and all subsequent figures, embryos are oriented with anterior to the left. In addition, embryos are oriented to show views of lateral, dorsal on top, (left image) and dorsal (right image) domains. The repression domains are outlined to the right of each image: DR = dorsal repression, DLR = dorsal lateral repression, and VR = ventral repression. The schematic depicts the chimeric CRM combinations used: (A) 1.4-kb ind CRM drives expression of lacZ a 5–7 cell lateral stripe representative of ind expression; (B) 0.5 kb eve.stripe3/7 CRM drives expression of lacZ in two anterior-posterior stripes representative of eve.stripe3/7 expression; (C) eve.stripe3/7-ind chimeric CRM drives expression of lacZ in a non-additive fashion showing repression of eve.stripe3/7 in dorsal, dorsal lateral, and ventral regions; (D) eve.stripe3/7-mut-A-box-ind chimeric CRM supports non-additive expression with repression of eve.stripe3/7 in dorsal and ventral regions but not dorsal lateral regions.