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. 2011 Dec 22;6(12):e29136. doi: 10.1371/journal.pone.0029136

Figure 4. The cap complex formation is inhibited by mTOR inactivation.

Figure 4

(A) REN cells were treated as indicated and total proteins were incubated with 7-Methyl GTP-Sepharose beads. Input is 10% of the purification. Cap binding proteins were analyzed by WB with anti-4E-BP1 and with anti-eIF4G. eIF4E shows equal amount of purified proteins. Rapamycin treatment induces 4E-BP1 binding to eIF4E and eIF4G is displaced from the complex. Densitometric analysis of eIF4G levels normalized to eIF4E are reported. (B) Densitometric analysis of eIF4G levels normalized to eIF4E, from 5 independent experiments are reported. (C) REN cells were treated as indicated and total proteins were incubated with 7-Methyl GTP-Sepharose beads. Input is 5% of the purification. Cap binding proteins were analyzed by WB with anti-4E-BP1 and with anti-eIF4G. eIF4E shows equal amount of purified proteins.