Figure 1. Generation of hepatocyte-specific Commd1 knockout mouse.

A.) Schematic representation of the Commd1 gene-targeting strategy to generate a hepatic-specific Commd1 knockout mouse, including a map of the COMMD1 exon 1 allele and the targeting vector with loxP sites (solid boxes), FRT sites (open boxes), and neomycin selection gene (Neo). Homologous recombination is marked with dotted lines. Hepatocyte-specific deletion of Commd1 was accomplished by cross-breeding of Commd1 ^loxP/loxP mice with Alb-Cre mice. This resulted in the generation of Commd1 ^Δhep mice (null allele). The locations of the PCR primer (P1, P2 and P3) binding sites used for genotyping are shown as open arrows. (Expected fragments: WT: 500 bp, LoxP: 350 bp, Null: 300 bp) B.) PCR analysis of liver tissue DNA of Commd1 WT, WT/loxP, loxP/loxP and Null/WT mice at six weeks of age. C is a negative control (H₂O). C.) Immunoblot analysis of Commd1 expression in liver tissue of Commd1 ^loxP/loxP and Commd1 ^Δhep mice. 30 µg tissue homogenates were analyzed by SDS-PAGE, and immunoblotted (IB) for Commd1 and α-Tubulin expression.