Table 2. Y3H analysis of protein-protein interactions between the Had heterodimers and the FAS-II proteins.
| AD fusions | BD gene fusions and pmet operon fusions | ||||||||
| 1 | 2 | 3 | 4 | 5 | |||||
| BD-lam | BD-HadA | BD-HadC | BD-HadC | BD-HadA | |||||
| pmet-HadB | pmet-HadB | pmet-HadA | pmet-HadC | ||||||
| Meta | Met | Met | Met | ||||||
| Ø | -b | - | - | - | - | - | - | - | - |
| mabA | - | - | - | - | - | - | - | - | - |
| inhA | - | - | - | - | - | - | - | - | - |
| kasA | - | +/- | + | - | - | - | - | - | - |
| kasB | - | - | +/- | +/- | +/- | - | - | - | - |
| mtfabH | - | - | +/- | - | - | - | - | - | - |
| mtfabD | - | - | - | - | - | - | - | - | - |
a
Met Indicates the presence of 1mM methionine in the medium.
b
Each sign symbolizes the growth on three selective media (DOBA-LTH, DOBA-LTA, and DOBA-LTHA).The rules of attribution of either +, +/-, or - are given in Materials and Methods.