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. 2011 Dec 22;6(12):e29525. doi: 10.1371/journal.pone.0029525

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Tzivelekidis et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Aminoacyl-tRNA stability towards hydrolysis was determined with 3 µM yeast eEF1A (yEF1A, filled circles) or glucosylated yeast eEF1A (yEF1A, open circles), 1 mM GTP and 1 µM [¹⁴C]Phe-tRNA^Phe for 60 min. As a control [¹⁴C]Phe-tRNA^Phe was incubated without eEF1A (triangles). Non-hydrolyzed [¹⁴C]Phe-tRNA^Phe was quantified by filter binding assay and scintillation counting.