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. 2011 Dec 22;6(12):e29450. doi: 10.1371/journal.pone.0029450

Figure 3. Validation of genes with DMRs in their promoters by MS-PCR and bisulfite sequencing.

Figure 3

A. Real-time PCR showing the differential expression of selected genes between the cisplatin sensitive A2780 and the cisplatin resistant A2780CP cells. B. MS-PCR data showing differential methylation states in promoter regions of the selected genes between A2780 and A2780CP cells. C. Bisulfite sequencing result for promoter regions of selected gene PTK6, PRKCE and BCL2L1 (white cycle: unmethylated CpG, Black cycle: methylated CpG).