Figure 1. Characterization of progenitor epithelial cells isolated from pig bone marrow.
(A) Colony morphology of progenitor epithelial cells derived from bone marrow. (B) Expression of stem cells markers on progenitor epithelial cells. Epithelial colony cells from primary cell cultures were examined for the expression of lung and other stem cell markers by using specific antibodies directed against Ccsp, Oct4 and SSEA-1. (C) Expression of epithelial cell markers on progenitor epithelial cells. Primary cell cultures were examined for the expression of specific epithelial cell markers: Pan-K, K-18, and occludin. (D–F) Absence of non-specific binding by progenitor epithelial cells. The cells were incubated with FITC-labeled secondary antibodies (D) goat anti-rabbit (E) goat anti-mouse (F) donkey anti-goat antibodies. Nuclei were stained by DAPI.