Table 6. Transition states (expression levels) during reprogramming using the five experimental recipes plus GATA6 knockdown.
Gene | Differentiation Attractor | Differentiation Barrier | Valley at step 2 | hES Barrier at step 3 | hES Valley at step 4,5,6 | hES Attractor at 7 |
OCT4 | 0 | 1 | 1 | 1 | 1 | 1 |
SOX2 | 0 | 1 | 1 | 1 | 1 | 1 |
NANOG | 0 | 0 | 0 | 1 | 1 | 1 |
Oct4-Sox2 | 0 | 0 | 1 | 1 | 1 | 1 |
KLF4 | 0 | 0.5 | 1 | 1 | 1 | 1 |
FOXD3 | 0 | 0 | 0.547 | 0.51 | 1 | 1 |
ZIC3 | 0 | 0 | 0.5022 | 0.4843 | 1 | 1 |
ZFP42 | 0 | 0 | 0.5098 | 0.4943 | 1 | 1 |
GDF3 | 0 | 0 | 0 | 0 | 1 | 1 |
TDGF1 | 0 | 0 | 1 | 1 | 1 | 1 |
PBX1 | 0 | 0 | 0 | 0 | 1 | 1 |
GATA2 | 1 | 1 | 0.5186 | 0.4915 | 0.16 | 0 |
GATA3 | 1 | 1 | 1 | 1 | 0 | 0 |
hCGb | 1 | 1 | 0 | 0 | 0 | 0 |
hCGa | 1 | 1 | 0.5153 | 0.4843 | 0.4 | 0 |
CDX2 | 1 | 1 | 1 | 1 | 0 | 0 |
GATA4 | 1 | 1 | 0.546 | 0.4858 | 0 | 0 |
GATA6 | 1 | 0 | 0 | 0 | 0 | 0 |
FOXA2 | 1 | 1 | 0.5033 | 0.7137 | 0.4 | 0 |
AFP | 1 | 1 | 0.5405 | 0.3604 | 0.6 | 0 |
SOX17 | 1 | 1 | 0.4978 | 0.463 | 0.32 | 0 |
T | 1 | 1 | 0.5142 | 0.4843 | 0 | 0 |