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. 2011 Dec 22;6(12):e29566. doi: 10.1371/journal.pone.0029566

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Ludgate et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A. Full-length DHBc proteins or firefly luciferase were translated in RRL and interaction with B23 or I2PP2A purified from HEK293T cells was determined by coimmunoprecipitation. Lanes 1–7 show RRL-translated input proteins (autoradiogram). Lanes 8–14 show the B23 immunoprecipitate (autoradiogram, top panel, and CB stain, bottom panel) and lanes 15-21 show the I2PP2A immunoprecipitate (autoradiogram, top panel, and CB stain, bottom panel). B. GST-DCC fusion proteins were translated in RRL and interaction with B23 or I2PP2A purified from HEK293T cells was determined by coimmunoprecipitation. The WT DCC196 fusion protein was either dephosphorylated with CIAP or left untreated. Lanes 1–6 show RRL-translated input proteins. Lanes 7–12 show the B23 immunoprecipitate (autoradiogram, top panel, and CB stain, bottom panel) and lanes 13–18 show the I2PP2A immunoprecipitate (autoradiogram, top panel, and CB stain, bottom panel). I2, I2PP2A-Flag-HA.