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. Author manuscript; available in PMC: 2013 Jan 1.
Published in final edited form as: Hepatology. 2011 Dec 6;55(1):222–232. doi: 10.1002/hep.24690

Figure 3. APAP induces autophagic flux and suppresses mTOR in primary mouse hepatocytes.

Figure 3

Figure 3

(A) Ad-GFP-LC3 infected hepatocytes were treated with APAP (5 mM) in the absence or presence of CQ (20 µM) for 6 hrs and examined by fluorescence microscopy. Scale bar: 20 µm. (B) GFP-LC3 puncta (mean ± SEM) were quantified for each experiment (n=3). At least 30 cells were counted in each individual experiment *: p<0.01. (C)Total lysates were subjected to immunoblot assay for LC3 and p62. (D) Primary mouse hepatocytes were treated with different concentrations of APAP (0, 1.25, 2.5, 5 & 10 mM) for 6 hrs. Total cellular lysates were subjected to immunoblot assay.