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. Author manuscript; available in PMC: 2013 Jan 1.
Published in final edited form as: Hepatology. 2011 Dec 6;55(1):222–232. doi: 10.1002/hep.24690

Figure 5. APAP induces necrotic cell death in primary mouse hepatocytes.

Figure 5

(A) Representative photographs of hepatocytes immunostained with an anti-HMGB1 antibody for cells either non-treated (panels a–d) or treated with APAP (10 mM, panels e–h) for 24 hrs. Cell nuclei were stained with Hoechst 33342. Panels d and h are enlarged photographs from the boxed areas from panels c and g respectively. (B) Cells with released HMGB1 from the nuclei were quantified for each experiment (n=3). More than 300 cells were counted in each individual experiment *: p<0.01.