Figure 1.

Efficient muscle-specific deletion of IKK2 in IKK2 mutant mice. (a) The upper panel shows a Western blot analysis of protein extracts prepared from control and conditionally IKK2-deficient mice (lanes 1–7) and from mouse embryonic fibroblasts of control (lane 8) and conventional IKK2-deficient mice (lane 9) probed with an anti-IKK2–specific antiserum. The genotypes of the mice are as follows: IKK2^WT, lanes 1 and 5; IKK2^Het, lanes 2 and 6; IKK2^Mus, lane 3; IKK2^Het/Mus, lanes 4 and 7. “NS” marks the position of a nonspecific band detected by the antiserum in skeletal muscle. The lower panel shows a Western blot analysis of the same blot shown in the upper panel, after stripping and reprobing with an anti-actin–specific antiserum. (b) Western blot analysis of IKK1 expression in skeletal muscle of different IKK2 mutant mice. The genotypes of the mice are as follows: IKK2^WT, lanes 1 and 5; IKK2^Het, lanes 2 and 6; IKK2^Mus, lane 3; IKK2^Het/Mus, lane 4. (c) Western Blot analysis of IKK2 expression in adipose tissue of different conditional IKK2 mutant mice. The genotypes of the mice are as follows: IKK2^WT, lanes 1 and 5; IKK2^Het, lanes 2 and 6; IKK2^Mus, lane 3; IKK2^Het/Mus, lane 4.