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. Author manuscript; available in PMC: 2013 Jan 1.
Published in final edited form as: J Cell Biochem. 2012 Jan;113(1):31–38. doi: 10.1002/jcb.23324

FIGURE 5. Impact of Lrp5/6 inactivation on small intestine differentiation.

FIGURE 5

FFPE sections of small intestine from E17.5 wild-type (A–C) or double mutants (D–F) stained with NBT/BCIP to detect enterocyte-specific ALP activity (A,D), Grimelius (enteroendocrine cell–specific) (B,E), or alcian blue (goblet cell–specific)(C,F). NBT/BCIP staining of the epithelium was much stronger in the double mutant (D) than in the wild type (A). Note strong staining in the intervillus region of the double mutant (arrow). Enteroendocrine cells were almost completely absent in double-mutant intestine (E) relative to wild-type intestine (B). Goblet cells were present in both wild-type (C) and double-mutant (F) intestine. Scale bars indicate 50 µm.